Bioremediation Products for In Situ Soil & Groundwater Cleanup

Bioaugmentation Cultures

Newman Zone SDC-9

Bioaugmentation culture for groundwater remediation

SDC-9 is a field-proven consortium of microorganisms used for in situ bioremediation of chlorinated solvents. It contains multiple strains of Dehalococcoides mccartyi (Dhc), the only species known to completely biodegrade PCE and TCE to non-toxic ethene.

For sites where Dhc are absent or present at low concentrations, SDC-9 provides the bacteria needed for complete dechlorination. Even where Dhc is already present, bioaugmentation can improve dechlorination rates, use electron donor more efficiently, and help reach site closure sooner.

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SDC-9 canister

Why Newman Zone SDC-9

Successful reductive dechlorination depends on having the right microorganisms in place. SDC-9 is designed to introduce and support the microbial population needed to complete dechlorination of chlorinated solvents, especially where native Dhc are absent or insufficient.

Because SDC-9 is a mixed culture rather than a pure isolate, it includes beneficial organisms that support Dhc growth by supplying substrates and growth factors. This helps shorten lag time and increase dechlorination rates compared with relying on native populations alone.

Complete Dechlorination

Provides multiple strains of Dehalococcoides mccartyi needed to fully convert chlorinated solvents to ethene.shear mixing or during injection, eliminating the need for specialized equipment.

Higher Dechlorination Rates

Contains a natural consortium of supporting microbes that help Dhc grow faster and dechlorinate more efficiently.

Low pH Tolerant

Continues to perform at pH as low as 5.5, although pH above 6.0 is recommended for better dechlorination.

Field-Proven Culture

Used for in situ bioaugmentation where native Dhc are absent, low, or not performing adequately

Technical Details

How it Works

1. Introduces the Required Microbes
SDC-9 adds multiple strains of Dehalococcoides mccartyi (Dhc) and other beneficial microorganisms to the treatment zone.

2. Supports Reductive Dechlorination
These microbes use anaerobic conditions and available electron donor to biologically reduce chlorinated compounds stepwise toward ethene.

3. Improves Treatment Performance
By increasing the active dechlorinating population, SDC-9 can accelerate treatment, improve donor efficiency, and support complete dechlorination.

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Application & Handling

  • Commonly injected between rounds of anaerobic water and electron donor
  • This approach minimizes oxygen exposure while helping distribute SDC-9 throughout the treatment area
  • Recommended dosing is 1 × 10⁷ Dhc cells per liter in target zone

Contaminants Treated:

PCE, TCE, cis-DCE, trans-DCE, 1,1-DCE, Vinyl chloride, Freon 11, Freon 113, 1,1,2,2-Tetrachloroethane, 1,1,1-Trichloroethane, 1,1-Dichloroethane, Carbon tetrachloride, Chloroform, Dichloromethane, HCFC, Tetrafluoroethene

Culture Characteristics

  • Cell count: >1 × 10¹¹ Dhc cells/L
    pH: 6.0–8.0
  • Density: 0.9–1.1 g/cm³
  • Appearance: light greenish, murky liquid
  • Odor: musty

Packaging & Storage

  • 19 L stainless steel kegs
  • Pressurized with nitrogen
  • Calibrated delivery systems provided

Storage:

  • Store at 2–4°C
  • Shelf life up to 3 weeks
  • Avoid freezing
  • Avoid exposure to oxygen

Safety

Non-toxic, non-pathogenic, non-genetically modified microbial consortium. No known hazards under normal use conditions. PPE recommended during handling.

Need help planning a bioaugmentation program?

Talk with our team about dosing, injection sequencing, and integrating SDC-9 with anaerobic water and electron donors.

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